Open AccessFast Screening for the Purification of Proteins Using Membrane Adsorber Technology
Author(s) -
Harkensee D.,
Kökpinar Ö.,
Walter J.,
Kasper C.,
Beutel S.,
Reif O.W.,
Scheper T.,
Ulber R.
Publication year - 2007
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200720194
Subject(s) - membrane , chromatography , chemistry , protein purification , ligand (biochemistry) , membrane protein , affinity chromatography , ion exchange , lysozyme , glycoprotein , downstream processing , biochemistry , ion , enzyme , organic chemistry , receptor
New membrane systems for the purification of proteins are presented. These disposable devices enable time saving screenings of downstream conditions with a small amount of protein solution. Furthermore, scaling up to the production level is directly possible since devices with effective membrane surfaces from 10 cm 2 to 1 m 2 are available. Several membranes like ion exchange membranes were investigated for the determination of protein binding capacities. Metal chelate membranes were used for ligand binding, which enables the determination of the membrane systems potential for affinity chromatography. Lysozyme and BSA are chosen for the determination of binding capacities of ion exchange membranes. Furthermore, Con A membranes, suitable for the isolation of glycoproteins, were developed and their binding properties were tested.