Open AccessEnhanced Biosynthesis of Sulfide Oxidase by Arthrobacter Species Using Response Surface Methodology
Author(s) -
Mohapatra B. R.,
Gould W. D.,
Dinardo O.,
Papavinasam S.,
Koren D. W.,
Revie R. W.
Publication year - 2007
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200620190
Subject(s) - yeast extract , response surface methodology , central composite design , arthrobacter , fermentation , sulfide , biochemistry , yeast , chemistry , food science , glucose oxidase , factorial experiment , enzyme , chromatography , organic chemistry , statistics , mathematics
Response surface methodology was used to develop a fermentation medium for the enhanced biosynthesis of a novel sulfide oxidase by Arthrobacter species strain FR‐3. The interactive effect of the medium components – such as glucose as the carbon source, and tryptone and yeast extract as the nitrogen source – was evaluated by a 2 3 ‐factorial central composite statistical design. Glucose and yeast extract were found to be the more influential medium constituents compared to tryptone since they had lower coefficients of linear effect, P ‐values (< 0.02). The optimal fermentation medium components for the enhanced production of sulfide oxidase were recorded as glucose (8.98 g/L), tryptone (10.62 g/L) and yeast extract (7.3 g/L). Optimization of the medium constituents increased the experimental enzyme yield by 54 % compared to the unoptimized medium. This is the first report on the overproduction of sulfide oxidase by using response surface methodology.