Open AccessHigh Frequency in vitro Multiplication of Centella asiatica : An Important Industrial Medicinal Herb
Author(s) -
Sivakumar G.,
Alagumanian S.,
Rao M. V.
Publication year - 2006
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200620159
Subject(s) - centella , shoot , explant culture , murashige and skoog medium , herb , acetic acid , botany , biology , micropropagation , medicinal herbs , in vitro , horticulture , traditional medicine , medicine , biochemistry
An efficient protocol was developed for rapid clonal propagation of the important medicinal plant, Centella asiatica (L.) Urban, using shoot tip culture. High frequency bud break (88 %) and multiple shoot formation (16.8 shoots/shoot tip) were induced from a shoot tip segment, which was cultured on MS medium supplemented with 6‐benzylaminopurine (BAP) (17.76 μM) and gibberillic acid (GA 3 ) (1.44 μM). Half‐strength Murashige and Skoog (MS) medium supplemented with naphthalen acetic acid (NAA) (10.74 μM) induced the maximum (27.66) number of roots. Plantlets with 3–4 fully expanded leaves and well‐developed roots were successfully transferred to potted soil which exhibited a 95 % survival. The protocol enables the harvest of more than 25,000 plantlets within 160 days starting from a single shoot tip explant.