Open AccessIntegrated Enzymatic Synthesis and Adsorption of Isomaltose in a Multiphase Fluidized Bed Reactor
Author(s) -
Ergezinger M.,
Bohnet M.,
Berensmeier S.,
Bucholz K.
Publication year - 2006
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200620151
Subject(s) - isomaltose , chemistry , adsorption , dextransucrase , fluidized bed , glucose 6 phosphate isomerase , immobilized enzyme , chromatography , packed bed , chemical engineering , maltose , kinetics , sucrose , enzyme , organic chemistry , lactic acid , biology , bacteria , engineering , genetics , leuconostoc mesenteroides , physics , quantum mechanics
Dextransucrase catalyzes the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. A process on a technical scale with immobilized enzyme was established to produce isomaltose, a disaccharide of industrial interest. Isomaltose is also a reactant for dextransucrase and has to be quickly taken out of the reaction solution. This was realized by integrated adsorption of isomaltose on zeolites. In the case of biotransformation the reactor works with a fluidized bed of immobilized enzyme and the in situ separation is realized with a suspension flow of adsorbent. This process was investigated experimentally and theoretically. With a design model consisting of hydrodynamics, kinetics of enzymatic synthesis, and thermodynamics of adsorption, a comparison was made between experimental and calculated data.