Open AccessProduction of Sophorolipids from Candida bombicola ATCC 22214 Using Turkish Corn Oil and Honey
Author(s) -
Pekin G.,
VardarSukan F.,
Kosaric N.
Publication year - 2005
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200520086
Subject(s) - food science , corn oil , corn steep liquor , chemistry , vegetable oil , carbon source , hexane , chromatography , fermentation , biochemistry
The ability of Candida bombicola ATCC 22214 to produce sophorolipids using Turkish corn oil and honey was investigated. Shake flask experiments were carried out both with and without the addition of glucose as the second carbon source. The organism could produce sophorolipids under both conditions but higher production was obtained when corn oil was combined with glucose. The 3 L bioreactor was first operated in batch mode, using both corn oil and glucose. When all the glucose was consumed, 1 / 4 th of the broth was pumped out and was replaced by freshly prepared medium containing 10 % [w/v] of cheap market honey as the sole carbon source. Feed was comprised of corn oil. High concentrations of sophorolipids (> 400 g/L) were produced. The crude products obtained from the batch cultivation could be solidified as very light brown solids when unused oil was removed by hexane, while the products of the two‐stage cultivation remained as viscous, honey‐like liquids after identical treatments.