Open AccessCharacteristics of Helianthus annuus Plant Cell Culture as a Producer of Immunologically Active Exopolysaccharides
Author(s) -
Pavlov A.,
Werner S.,
Ilieva M.,
Bley T.
Publication year - 2005
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200420074
Subject(s) - helianthus annuus , sunflower , plant cell , biology , botany , plant growth , cell culture , suspension culture , tissue culture , in vitro , biochemistry , horticulture , genetics , gene
The aim of this work was to determine relationships between the growth and biosynthesis of exopolysaccharides by sunflower ( Helianthus annuus 1805) plant cell culture and the uptake of the main compounds from the nutrient medium through the study of their time courses. It was established that plant cell culture biosynthesized 12.8 g/L biomass and 4.3 g/L exopolysaccharides. The calculated maximum specific growth rate and doubling time were μ max = 0.21 d –1 and t d = 3.31 d, respectively. The productivity of exopolysaccharides in the plant in vitro system was found to be 0.43 g/(L × d). Data regarding the physiology of Helianthus annuus 1805 plant cell culture are presented. On the basis of the achieved results it can be concluded that the plant cell suspension of Helianthus annuus 1805 is a good producer of exopolysaccharides which exhibit immunostimulating activity.