Open AccessRapid Purification of High Quality DNA from Large Whole Blood Samples Using a New Rapid Protocol
Author(s) -
Long J.R.,
Zhang Y.Y.,
Hofer E.,
Seemann C.,
Schatz M.,
O'Brien D.,
Deng H.W.
Publication year - 2004
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200420040
Subject(s) - dna , protocol (science) , genomic dna , isolation (microbiology) , dna extraction , computational biology , biology , whole blood , chromatography , chemistry , polymerase chain reaction , genetics , bioinformatics , gene , medicine , immunology , pathology , alternative medicine
The efficiency and quality of DNA purification from blood samples is one of the important issues for genetic studies on human diseases. Several protocols for the isolation of genomic DNA from blood have been described. In order to improve the efficiency of the purification without affecting DNA quality and quantity, a new protocol, called Puregene EP (Enhanced Productivity) is described for this kit.