Open AccessStudy of Extracellular Plant Lactase
Author(s) -
Neubert K.,
Stano J.,
Mičieta K.,
Koreňová M.,
Blanáriková V.
Publication year - 2004
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200420031
Subject(s) - extracellular , lactase , substrate (aquarium) , hydrolysis , galactose , chemistry , biochemistry , food science , enzyme , chromatography , biology , ecology
Using a synthetic substrate, a simple and sensitive procedure for the determination of extracellular lactase was developed. The enzyme studied produced by the tested plant material hydrolyzed the substrate (1‐naphtyl‐α‐ D ‐galactopyranoside) to α‐ D ‐galactose and 1‐naphthol. By simultaneous azocoupling of 1‐naphthol hardly water‐soluble azo‐dyes were produced. The evaluation of the intensity of dyed zones allowed the extracellular lactase activity to be assessed. The agar plate method described permitted rapid, simple, and specific detection of plant producers of extracellular lactase and proved to be perspectively useful in inhibitory and/or biotechnological studies.