Open AccessReconfigurable Implication and Inhibition Boolean logic gates based on NAD + ‐dependent enzymes: Application to signal‐controlled biofuel cells and molecule release
Author(s) -
Bollella Paolo,
Kadambar Vasantha Krishna,
Melman Artem,
Katz Evgeny
Publication year - 2022
Publication title -
electrochemical science advances
Language(s) - English
Resource type - Journals
ISSN - 2698-5977
DOI - 10.1002/elsa.202100008
Subject(s) - logic gate , nad+ kinase , signal (programming language) , enzyme , biomolecule , and gate , chemistry , or gate , computer science , nanotechnology , materials science , biochemistry , algorithm , programming language
The Implication and Inhibition Boolean logic gates were realized using NAD + /NADH‐dependent dehydrogenases combined with hexokinase competing for biomolecule input signals. Both logic gates operated with the same enzyme composition and their reconfiguration was achieved simply by redefining the input signals. The output signals produced by the logic gates were analyzed optically and electrochemically, particularly using enzyme‐modified electrodes. The logically processed input signals were used to switch operation of a biofuel cell and activate a molecule release process.