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Overview of in‐capillary enzymatic reactions using capillary electrophoresis
Author(s) -
Rozenski Jef,
Asfaw Adissu Alemayehu,
Van Schepdael Ann
Publication year - 2022
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202100161
Subject(s) - capillary electrophoresis , homogeneous , capillary action , laminar flow , chromatography , microanalysis , chemistry , capillary electrochromatography , materials science , organic chemistry , mechanics , physics , composite material , thermodynamics
This review summarizes the research that has recently been performed on in‐capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At‐inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non‐magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.