Premium
Front cover: Concentration effects on capture rate and translocation configuration of nanopore‐based DNA detection
Author(s) -
Zhang Yin,
Zhao Jiabin,
Kan Yajing,
Ji Rui,
Pan Jianqiang,
Huang Weichi,
Xu Zheng,
Si Wei,
Sha Jingjie
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202070091
Subject(s) - nanopore , dna , molecule , linearity , nanopore sequencing , cover (algebra) , chemistry , nanotechnology , materials science , biophysics , analytical chemistry (journal) , chemical physics , chromatography , dna sequencing , biology , biochemistry , physics , mechanical engineering , organic chemistry , quantum mechanics , engineering
DOI: 10.1002/elps.202000016 The cover picture shows the process of two DNA molecules electrophoretically co‐transporting through a solid‐state nanopore, i.e., the second DNA molecule places its end into the pore before the first DNA molecule goes out of the pore. Considerable cotranslocation events have been detected in the case of relatively large‐sized nanopores and high applied voltage. Furthermore, the percentage of cotranslocation events increases with DNA concentration, which leads to the relationship between capture rate and DNA concentration deviating from linearity. In order to accurately measure DNA concentration by nanopores, the capture rate should be corrected by double‐counting these kinds of cotranslocation events.