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Integrated dielectrophoretic and impedimetric biosensor provides a template for universal biomarker sensing in clinical samples
Author(s) -
Velmanickam Logeeshan,
Jayasooriya Vidura,
Nawarathna Dharmakeerthi
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000347
Subject(s) - biosensor , dielectrophoresis , point of care testing , biomarker , point of care , nucleic acid , nanotechnology , chromatography , detection limit , materials science , chemistry , computational biology , microfluidics , biology , medicine , biochemistry , pathology , immunology
The detection and quantification of nucleic acid and proteomic biomarkers in bodily fluids is a critical part of many medical screening and diagnoses. However, majority of the current detection platforms are not ideal for routine, rapid, and low‐cost testing in point‐of‐care settings. To address this issue, we developed a concept for a disposable universal point‐of‐care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples. The central tenet of sensing is the use of dielectrophoresis, electrothermal effects, and thermophoresis to selectively and rapidly isolate the biomarkers of interest in electrodes and then quantify using electrical impedance. When the sensor was applied to quantify microRNA and antigen biomarker molecules directly in diluted serum samples, it produced a LOD values in the fM range and sensitivity values from 10 12 to 10 15 Ω/M with a 30 min assay time and assay cost of less than $50 per assay.