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Capillary electrophoretic method for Staphylococcus aureus detection by using a novel antimicrobial peptide
Author(s) -
Hong Tingting,
Qiu Lin,
Rui Wen,
Li Ying,
Zheng Ronghui,
Guo Qianqian,
Zhou Shuwen,
Cui Pengfei,
Sheng Jingyu,
Ni Xinye,
Wang Jianhao,
Jiang Pengju
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000323
Subject(s) - staphylococcus aureus , capillary electrophoresis , peptide , antimicrobial , chemistry , microbiology and biotechnology , gelatinase , chromatography , staphylococcus , biochemistry , bacteria , biology , enzyme , genetics
A novel peptide containing antimicrobial sequence and gelatinase cleavage sites was designed for Staphylococcus aureus detection. Since Staphylococcus aureus could secrete gelatinase, the fluorescein labeled peptide GKRWWKWWRRPLGVRGC could be recognized and cleaved. The obtained products were able to be analyzed by capillary electrophoresis with fluorescence detection. To explore the effect of Staphylococcus aureus concentration on enzyme digestion ability of peptide, Staphylococcus aureus with different concentrations were incubated with the peptide. Results indicated that capillary electrophoretic method was efficient for determining Staphylococcus aureus content. Compared with traditional approaches for Staphylococcus aureus detection, capillary electrophoresis possessed higher efficiency, enhanced sensitivity, and low sample consumption. Moreover, the proposed peptide also presented desirable antimicrobial activity. It suggested that the novel antimicrobial peptide used in this research opens a new path of detecting Staphylococcus aureus by capillary electrophoretic method.