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Exploitation of a novel slowly mutating Y‐STRs set and evaluation of slowly mutating Y‐STRs plus Y‐SNPs typing strategy in forensic genetics and evolutionary research
Author(s) -
Liu Jing,
Ming Tianyue,
Lang Min,
Liu Hai,
Xie Minkun,
Li Jienan,
Wang Mengge,
Song Feng,
He Guanglin,
Wang Shouyu,
Wang Zheng,
Hou Yiping
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000302
Subject(s) - microsatellite , phylogenetic tree , biology , pedigree chart , genetics , haplotype , mutation rate , population , evolutionary biology , population genetics , gene , genotype , allele , demography , sociology
The Y‐chromosome short tandem repeats (Y‐STRs) loci with different mutation rates existing in the Y chromosome non‐recombination region (NRY) allow to be applied in human forensics, genealogical researches, historical investigations and evolutionary studies. Currently, there is a high demand for pedigree search to narrow the scope of crime investigations. However, the commonly used Y‐STRs kits generally contain Y‐STRs with high mutation rates that could cause individuals from the same pedigree to display different haplotypes. Herein, we put forward a new strategy of Slowly Mutating (SM) Y‐STRs plus Y‐SNPs typing, which could not only improve the resolution and accuracy of pedigree search, but also be applicable to evolutionary research. First, we developed a nine SM Y‐STRs assay by evaluating their mutation rates in 210 pedigrees. Then the gene diversity and efficiency of the SM Y‐STRs and 172 Y‐SNPs sets were investigated by 2304 unrelated males from 24 populations. Furthermore, network and time estimation analyses were performed to evaluate the new strategy's capability to reconstruct phylogenetic tree and reliability to infer the time to the most recent common ancestor (TMRCA). The nine SM Y‐STRs assay even had a higher resolution and a comparable capacity of revealing population genetic differentiation compared to 172 Y‐SNPs system. This new strategy could optimize the phylogenetic tree generated by commonly used Y‐STR panels and obtain a quite consistent time estimations with the published dating.