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Determination of complex type free, non‐conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency
Author(s) -
Mátyás Bence,
Singer Júlia,
Szarka Máté,
Lowy Daniel A.,
Döncző Boglárka,
Makleit Péter,
FailocRojas Virgilio E.,
Ramirez Andrés,
Martínez Pedro,
Sándor Zsolt,
Kincses Ida,
Guttman András
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000254
Subject(s) - glycan , xylem , chemistry , nutrient , oligosaccharide , conjugated system , nutrient deficiency , polysaccharide , biochemistry , capillary electrophoresis , botany , food science , chromatography , biology , organic chemistry , glycoprotein , polymer
Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown. Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported. While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF). Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).