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Quantification of the bisphosphonate alendronate using capillary electrophoresis mass spectrometry with dynamic pH barrage junction focusing
Author(s) -
Zhao Tingting,
Wang Lingyu,
Li Yueyang,
Chen Sijia,
Wang Rizhi,
Chen David Da Yong
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000228
Subject(s) - derivatization , chromatography , capillary electrophoresis , chemistry , capillary electrophoresis–mass spectrometry , electrospray , stacking , mass spectrometry , analytical chemistry (journal) , detection limit , bisphosphonate , electrospray ionization , osteoporosis , medicine , organic chemistry , endocrinology
A quantitative method was developed for the direct identity confirmation and quantification of alendronate using CE‐MS combined with a pH‐assisted focusing technique, dynamic pH barrage junction focusing. A pH‐induced variation in electrophoretic mobility led to online focusing of alendronate at the sample/pH barrage boundary, significantly improving the detection sensitivity. In addition, the use of a flow‐through microvial CE electrospray interface and the multiple reaction monitoring mode of MS further improved the specificity and quantification capability of this technology. This quantitative method presented a wide linear dynamic range over 8–2000 ng/mL and an LOD of 2 ng/mL. A 460‐fold improvement in sensitivity was obtained when pH barrage junction focusing was applied during the CE process, in comparison to when normal CE was conducted without online sample stacking. The superior detection sensitivity over previously reported methods enables direct analysis of bisphosphonate compounds, eliminating tedious pre‐column sample enrichment and derivatization. Validation of alendronate content in a commercial drug tablet further proved the reliability and power of this method. This simple method with no sample derivatization, superior sensitivity, and short run time (<8 min) is a promising alternative for accurate quantification of alendronate and other types of bisphosphonate compounds in both drug formulations and plasma samples.