Premium
Ultrahigh throughput beehive‐like device for blood plasma separation
Author(s) -
Jiang Fengtao,
Xiang Nan,
Ni Zhonghua
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000202
Subject(s) - throughput , microfluidics , materials science , plasma , microchannel , separation (statistics) , sample (material) , optoelectronics , nanotechnology , chromatography , computer science , chemistry , physics , telecommunications , quantum mechanics , machine learning , wireless
We report here a low‐cost, rapid‐prototyping, and beehive‐like multilayer polymer microfluidic device for ultrahigh‐throughput blood plasma separation. To understand the device physics and optimize the device structure, the effect of cross‐sectional dimension and operational parameter on particle focusing behavior was explored using a single spiral microchannel device. Then, the blood plasma separation performance of the determined channel structure was validated using the blood samples with different hematocrits (HCTs). It was found that a high separation efficiency of 99% could be achieved using the blood sample with an HCT of 0.5% at a high throughput of 1 mL/min. Finally, a multilayer microfluidic device with a novel beehive‐like multiplexing channel arrangement was developed for ultrahigh‐throughput blood plasma separation. The prototype device could be fabricated within ∼1 hour utilizing the laser cutting and thermal lamination methods. The total processing throughput could reach up to 72 mL/min for 0.5% HCT sample with a plasma separation ratio close to 90%. Our device may hold potentials for the ultrahigh‐throughput separation of blood plasma from large volume blood samples for downstream disease diagnosis.