Premium
An impedimetric assay for the identification of abnormal mitochondrial dynamics in living cells
Author(s) -
Galpayage Dona Kalpani N. U.,
Du E,
Wei Jianning
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000125
Subject(s) - huntingtin , mitochondrial fission , mitochondrial fusion , mitochondrion , microbiology and biotechnology , cytoplasm , biology , mitochondrial dna , organelle , biochemistry , gene , mutant
Mitochondrial dynamics (fission and fusion) plays an important role in cell functions. Disruption in mitochondrial dynamics has been associated with diseases such as neurobiological disorders and cardiovascular diseases. Analysis of mitochondrial fission/fusion has been mostly achieved through direct visualization of the fission/fusion events in live‐cell imaging of fluorescently labeled mitochondria. In this study, we demonstrated a label‐free, non‐invasive Electrical Impedance Spectroscopy (EIS) approach to analyze mitochondrial dynamics in a genetically modified human neuroblastoma SH‐SY5Y cell line with no huntingtin protein expression. Huntingtin protein has been shown to regulate mitochondria dynamics. We performed EIS studies on normal SH‐SY5Y cells and two independent clones of huntingtin‐null cells. The impedance data was used to determine the suspension conductivity and further cytoplasmic conductivity and relate to the abnormal mitochondrial dynamics. For instance, the cytoplasm conductivity value was increased by 11% from huntingtin‐null cells to normal cells. Results of this study demonstrated that EIS is sensitive to characterize the abnormal mitochondrial dynamics that can be difficult to quantify by the conventional microscopic method.