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Dielectrophoretic ultra‐high‐frequency characterization and in silico sorting on uptake of rare earth elements by Cupriavidus necator
Author(s) -
Giduthuri Anthony T.,
Adekanmbi Ezekiel O.,
Srivastava Soumya K.,
Moberly James G.
Publication year - 2021
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000095
Subject(s) - cupriavidus necator , necator americanus , europium , materials science , chemistry , bacteria , biology , zoology , genetics , optoelectronics , luminescence , polyhydroxyalkanoates , helminths , ascaris lumbricoides
Abstract Rare earth elements (REEs) are widely used across different industries due to their exceptional magnetic and electrical properties. In this work, Cupriavidus necator is characterized using dielectrophoretic ultra‐high‐frequency measurements, typically in MHz range to quantify the properties of cytoplasm in C. necator for its metal uptake/bioaccumulation capacity. Cupriavidus necator , a Gram‐negative bacteria strain is exposed to REEs like europium, samarium, and neodymium in this study. Dielectrophoretic crossover frequency experiments were performed on the native C. necator species pre‐ and post‐exposure to the REEs at MHz frequency range. The net conductivity of native C. necator , Cupriavidus europium , Cupriavidus samarium , and Cupriavidus neodymium are 15.95 ± 0.029 μS/cm, 16.15 ± 0.028 μS/cm, 16.05 ± 0.029 μS/cm, 15.61 ± 0.005 μS/cm respectively. The estimated properties of the membrane published by our group are used to develop a microfluidic sorter by modeling and simulation to separate REE absorbed C. necator from the unabsorbed native C. necator species using COMSOL Multiphysics commercial software package v5.5.