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Improved STR analysis of degraded DNA from human skeletal remains through in‐house MPS‐STR panel
Author(s) -
Cho Sohee,
Shin KyoungJin,
Bae SuJin,
Kwon YeLim,
Lee Soong Deok
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000070
Subject(s) - str analysis , amplicon , dna , massive parallel sequencing , microsatellite , capillary electrophoresis , dna profiling , str multiplex system , biology , computational biology , genetics , typing , polymerase chain reaction , dna sequencing , microbiology and biotechnology , allele , gene
DNA analysis of degraded samples and low‐copy number DNA derived from skeletal remains, one of the most challenging forensic tasks, is common in disaster victim identification and genetic analysis of historical materials. Massively parallel sequencing (MPS) is a useful technique for STR analysis that enables the sequencing of smaller amplicons compared with conventional capillary electrophoresis (CE), which is valuable for the analysis of degraded DNA. In this study, 92 samples of human skeletal remains (70+ years postmortem) were tested using an in‐house MPS‐STR system designed for the analysis of degraded DNA. Multiple intrinsic factors of DNA from skeletal remains that affect STR typing were assessed. The recovery of STR alleles was influenced more by DNA input amount for amplification rather than DNA degradation, which may be attributed from the high quantity and quality of libraries prepared for MPS run. In addition, the higher success rate of STR typing was achieved using the MPS‐STR system compared with a commercial CE‐STR system by providing smaller sized fragments for amplification. The results can provide constructive information for the analysis of degraded sample, and this MPS‐STR system will contribute in forensic application with regard to skeletal remain sample investigation.