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Metal–organic framework assisted matrix solid‐phase dispersion microextraction of saponins using response surface methodology
Author(s) -
Zhang Rui,
Xu Nan,
Wang Yue,
Liu Xungao,
Wang Shuling,
Cao Jun
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000042
Subject(s) - chromatography , detection limit , extraction (chemistry) , response surface methodology , solid phase microextraction , matrix (chemical analysis) , chemistry , adsorption , elution , dispersion (optics) , materials science , mass spectrometry , gas chromatography–mass spectrometry , physics , organic chemistry , optics
An efficient and simple metal–organic framework (MOF) assisted matrix solid‐phase dispersion (MSPD) microextraction was developed for the extraction of the five saponins in P. ginseng leaves. The target analyses were detected by ultra high performance chromatography coupled with time‐of‐flight MS. Experimental conditions for MSPD microextraction were optimized by the Box–Behnken design of the response surface methodology. The optimal conditions were as follows: 20 mg adsorbent, 80% methanol–water solution for elution, 60 s grinding time, and the MOF‐808 as the adsorbent. With the final optimized method, the calibration curves for five saponins showed good linearity ( R 2 > 0.998) within range of 0.01–100 μg/mL. In addition, analytical recoveries ranged from 87.04 to 103.78%, with the RSD below 5%. The limit of detection and LOQ range from 0.087 to 0.114 μg/mL and 0.292 to 0.379 μg/mL, respectively. Compared with the traditional extraction method and published methods, the newly MOF‐assisted MSPD extract exhibited higher extraction efficiency, simpler operation, and provided a cleaner extract with low consumption of organic reagents that was applied for rapid evaluation and quality control of active compounds from plants.