Premium
Interaction of heparin and tetraarginine in capillary electrophoresis: Implication for analytical applications
Author(s) -
Krizek Tomas,
Molnarova Katarina,
Pavlu Vera,
Filounova Barbora,
Martinkova Eva
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000011
Subject(s) - heparin , capillary electrophoresis , chemistry , electrolyte , capillary action , chromatography , electrophoresis , analytical chemistry (journal) , materials science , biochemistry , electrode , composite material
Interactions between heparin and tetraarginine in an acidic background electrolyte were investigated in capillary electrophoresis. The results showed that tetraarginine and heparin form a stable complex that migrates toward the anode immediately after coming into contact. When a zone of tetraarginine at a mg/mL concentration level passes through a zone of heparin at a μg/mL concentration level, tetraarginine is gradually removed by the formation of the complex that migrates in the opposite direction, thereby decreasing the tetraarginine peak area. The variation of the tetraarginine peak area as a function of the unfractionated heparin concentration was linear within the range 2–20 μg/mL, which enables us to detect and determine heparin concentrations undetectable with a UV detector. The same behavior was confirmed for low molecular weight heparin.