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Performance of laterally elongated pillar array columns in capillary electrochromatography mode
Author(s) -
Baca Martyna,
Kryj Agata,
Naghdi Elahe,
Gelin Pierre,
Sukas Sertan,
Laha Priya,
Terryn Herman,
Ottevaere Heidi,
De Malsche Wim
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.202000001
Subject(s) - capillary electrochromatography , electrochromatography , capillary action , materials science , dispersion (optics) , pillar , aspect ratio (aeronautics) , optics , analytical chemistry (journal) , geometry , composite material , chromatography , chemistry , physics , structural engineering , engineering , mathematics
In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.