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Front cover: A novel microchip‐based imaged CIEF‐MS system for comprehensive characterization and identification of biopharmaceutical charge variants
Author(s) -
Mack Scott,
Arnold Don,
Bogdan Greg,
Bousse Luc,
Danan Lieza,
Dolnik Vladislav,
Ducusin MaryAnn,
Gwerder Eric,
Herring Chris,
Jensen Morten,
Ji Jennifer,
Lacy Steve,
Richter Claudia,
Walton Ian,
Gentalen Erik
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970201
Subject(s) - isoelectric focusing , chromatography , biopharmaceutical , chemistry , characterization (materials science) , microsecond , front cover , capillary action , nanotechnology , materials science , cover (algebra) , physics , optics , biology , biochemistry , genetics , composite material , enzyme , mechanical engineering , engineering
DOI: 10.1002/elps.201900325 The cover picture shows direct coupling of an imaged capillary isoelectric focusing (iCIEF) microchip to a high‐resolution MS detector for characterization of intact biotherapeutic protein charge variants. Real‐time monitoring of the focusing and chemical mobilization phases of the assay is performed using absorbance imaging of the entire separation channel at 280 nm. The integrated iCIEF‐MS assay enables analysis of multiple critical quality attributes for biotherapeutic proteins in a single 15‐minute assay and has potential applications in multiple drug development and bioproduction processes.