Premium
Back cover: Bacillus licheniformis peptidoglycan characterization by CZE–MS: Assessment with the benchmark RP‐HPLC‐MS method
Author(s) -
Boulanger Madeleine,
Delvaux Cédric,
Quinton Loïc,
Joris Bernard,
De Pauw Edwin,
Far Johann
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970176
Subject(s) - bacillus licheniformis , peptidoglycan , high performance liquid chromatography , chromatography , chemistry , elution , benchmark (surveying) , gradient elution , biology , biochemistry , cell wall , bacillus subtilis , bacteria , genetics , geodesy , geography
DOI: 10.1002/elps.201900147 The cover picture shows the hyphenation of CE‐MS for the characterization of peptidoglycan (PGN) fragments obtained after mutanolysin digestion. CE‐MS data were favorably compared to the reverse‐phase HPLC benchmark method. Both methods provided comparable data although HPLC eluted PGN fragments of higher molecular weights. Remarkably, CE‐MS baseline resolved the modified PGN fragments containing chemical modifications of interest, namely the amidation and deacetylation.