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Cover feature: CE‐SDS method development, validation, and best practice—An overview
Author(s) -
Sänger–van de Griend Cari E.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970158
Subject(s) - monoclonal antibody , cover (algebra) , capillary electrophoresis , chromatography , capillary action , feature (linguistics) , chemistry , electrophoresis , materials science , antibody , biology , genetics , philosophy , mechanical engineering , linguistics , engineering , composite material
DOI: 10.1002/elps.201900094 The cover picture shows an impression of the separation of monoclonal antibodies in capillary gel electrophoresis, also called CE‐SDS. The capillary is filled with an entangled polymer network solution, symbolized as the “gray spaghetti”. The monoclonal antibody, highlighted in the circle, is composed of two Heavy Chains in green and two Light Chains in red. Size‐based separation after denaturation is visualized by the smallest fragment moving fastest to the right, and the intact monoclonal antibody migrating slowest on the left. The other fragments fill the space in between, sorted by size.