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Front Cover: Discrimination of single‐stranded DNA homopolymers by sieving out G‐quadruplex using tiny solid‐state nanopores
Author(s) -
Si Wei,
Yang Haojie,
Sha Jingjie,
Zhang Yin,
Chen Yunfei
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970141
Subject(s) - nanopore , nanopore sequencing , nanotechnology , nanoscopic scale , materials science , dna , solid state , silicon nitride , chemistry , dna sequencing , layer (electronics) , biochemistry
DOI: 10.1002/elps.201800537 The cover picture shows the application of a nanopore sensor being developed as a promising single‐molecule technology for DNA sequencing at single‐base resolution. A tiny nanoscale silicon nitride nanopore was fabricated by transmission electron microscope, the fabricated nanopore is a bit larger than the diameter of a single‐stranded DNA but smaller than the dimension of G‐tetramers. Then the nanopore could be used to sieve out G tetramers making sure only single‐stranded DNA homopolymers could translocate through the nanopore. Interestingly, the DNA bases could be identified and differentiated by measuring the respective ionic current blockades.

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