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Outside Back Cover: Separation‐free single‐base extension assay with fluorescence resonance energy transfer for rapid and convenient determination of DNA methylation status at specific cytosine and guanine dinucleotide sites
Author(s) -
Fujita Keisuke,
Hashimoto Masahiko
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970012
Subject(s) - förster resonance energy transfer , fluorescence , guanine , primer extension , acceptor , cytosine , chemistry , dna , terminator (solar) , nucleotide , biochemistry , base sequence , physics , quantum mechanics , gene , ionosphere , astronomy , condensed matter physics
DOI: 10.1002/elps.201800144 The cover picture shows a separation‐free single base extension (SBE) assay based on fluorescence resonance energy transfer (FRET) for rapid detection of 5‐methylcytosine (mC). A single base‐extended primer forms, which is 5'‐Cy3‐ and 3'‐Cy5‐tagged. A Cy5‐labeled terminator is incorporated into the 3'‐end of the extension primer if the added terminator is complementary to the target nucleotide. The Cy3 donor and Cy5 acceptor are in close proximity in the SBE product. Illumination of the donor results in FRET and excitation of the Cy5 acceptor, generating fluorescence from the acceptor.