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Outside Front Cover: On‐line electroextraction in capillary electrophoresis: Application on the determination of glutamic acid in soy sauces
Author(s) -
Campos Camila D. M.,
Reyes Felix G. R.,
Manz Andreas,
da Silva José A. F.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201970011
Subject(s) - capillary electrophoresis , chromatography , chemistry , ethylene glycol , acetic acid , electrophoresis , electrolyte , extraction (chemistry) , phase (matter) , aqueous solution , sodium acetate , aqueous two phase system , biochemistry , organic chemistry , electrode
DOI: 10.1002/elps.201800203 An on‐line, single step electroextraction is demonstrated for capillary electrophoresis and microchip electrophoresis. The electroextraction employed an aqueous two‐phase system (ATPS) formed by one 500 kDa dextran‐rich phase added to the samples and a second 6 kDa poly(ethylene glycol)‐rich phase containing 5.0 mol/L acetic acid. The second phase acted as background electrolyte. The ATPS enabled electrically driven extraction of target compounds, while demonstrated to retain sodium ions. The ATPS electrodriven system was applied on the determination of glutamate in soy sauces samples.