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Challenges and applications of isotachophoresis coupled to mass spectrometry: A review
Author(s) -
Melzer Tanja,
Wimmer Benedikt,
Bock Stephanie,
Posch Tjorben Nils,
Huhn Carolin
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900454
Subject(s) - isotachophoresis , capillary electrophoresis , capillary electrophoresis–mass spectrometry , chromatography , mass spectrometry , chemistry , electrophoresis , capillary action , analytical chemistry (journal) , materials science , electrospray ionization , electrolyte , electrode , composite material
Electrophoretic separations are of growing interest to tackle complex analytical challenges. Nevertheless, capillary electrophoresis, as the most common mode, still suffers from insufficient detection limits due to low capillary loadability. ITP is of growing interest as preconcentration method for capillary electrophoresis and is also interesting to be applied as an independent analytical method. While mass spectrometric detection is common for capillary electrophoresis, the combination of ITP with MS is still a niche technique. In this work, we want to give an overview on isotachophoretic effects in CE‐MS and ITP‐MS methods, as well as coupling techniques of ITP with CE‐MS. The challenges and possibilities associated with mass spectrometric detection in ITP and its coupling to capillary electrophoresis are critically discussed.