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Determination of S ‐adenosylmethionine, S ‐adenosylhomocysteine, and methylthioadenosine in urine using solvent‐modified micellar electrokinetic chromatography
Author(s) -
Ivanov Alexander Vladimirovich,
Kruglova Mariya Petrovna,
Virus Edward Danielevich,
Bulgakova Polina Olegovna,
Vital'evich Grachev Sergei,
Kubatiev Aslan Amirkhanovich
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900364
Subject(s) - micellar electrokinetic chromatography , isobutanol , chromatography , chemistry , urine , analyte , peg ratio , solvent , detection limit , alcohol , biochemistry , finance , economics
Abstract A new approach for direct determination of S ‐adenosylmethionine (SAM), S ‐adenosylhomocysteine (SAH), and methylthioadenosine (MTA) in urine was developed based on MEKC by using SDS modified with isobutanol in the presence of PEG‐300. Analytes were first extracted with grafted phenylborononic acid. Using a 50 µm internal diameter silica capillary of 32 cm total length filled with 0.05 M SDS, 0.05 M H 3 PO 4 , 5% (v/v) isobutanol, and 10% (v/v) PEG‐300, LOQ of 0.15 µM for SAM and SAH, and 0.2 µM for MTA was reached. Accuracy was 92% for MTA, 109% for SAH, and 105% for SAM, intra‐ and interday imprecision were <2.5 and ≤3%, respectively. The total time of analysis for one sample was 10 min. Analysis of 30 urine samples from healthy volunteers showed that the median SAM and SAH levels were 12.1 and 0.73 µM, respectively. MTA levels, which were determined in urine for the first time (according to our data), were 0.43 µM, and these values correlated well with the SAM level ( r = 0.748, p < 0.01).