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Electro‐kinetically driven route for highly sensitive blood pathology on a paper‐based device
Author(s) -
Mani Naresh Kumar,
Das Sankha Shuvra,
Dawn Sayantan,
Chakraborty Suman
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900356
Subject(s) - sensitivity (control systems) , computer science , electric field , materials science , point of care , nanotechnology , biological system , biomedical engineering , optoelectronics , chemistry , electronic engineering , pathology , physics , medicine , biology , engineering , quantum mechanics
Enhancing the sensitivity of colorimetric detection in paper‐devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper‐based devices, as mediated by electro‐kinetic mechanisms. By directly employing blood plasma, we investigate the electro‐kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low‐cost paper‐based point‐of‐care devices deployed in resource‐limited settings.