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Ion‐induced PCR strategy for mercury detection
Author(s) -
Chen Gangyi,
Wang Wei,
Yuan Yi,
Dong Juan,
Cui Xin,
Huang Xin,
Deng Yun,
Tang Zhuo
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900324
Subject(s) - mercury (programming language) , chemistry , thymine , agarose , metal ions in aqueous solution , selectivity , chromatography , environmental chemistry , ion , dna , biochemistry , computer science , organic chemistry , catalysis , programming language
Mercury contamination is one of the most serious environmental problems. It can cause serious effects on the human health, such as case damage in the brain, nervous system, immune system, and kidney failure. Therefore, development of an accurate, sensitive, and simple operational detection method for mercury is very necessary. Herein, we report a new strategy for mercury ion detection based on commonly used PCR technique. High selectivity and sensitivity were achieved by the formation of the thymine‐Hg‐thymine (T‐Hg‐T) unnatural base pair at the 3’‐end of PCR primers. The detection results of PCR amplification in presence of mercury ion could be reported either by using agarose gel analysis or through real‐time fluorometric dye tracing for different detection purposes. To our knowledge, this study represents the first application of PCR based technique to the detection of metal ions.