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Rapid pre‐concentration of Escherichia coli in a microfluidic paper‐based device using ion concentration polarization
Author(s) -
Perera A.T.K.,
Pudasaini Sanam,
Ahmed Syed Shaheer Uddin,
Phan DinhTuan,
Liu Yu,
Yang Chun
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900303
Subject(s) - microfluidics , ballast , concentration polarization , materials science , fluorescence , membrane , electrode , ion , analytical chemistry (journal) , voltage , chromatography , chemistry , nanotechnology , optics , ecology , biochemistry , physics , organic chemistry , quantum mechanics , biology
We report a microfluidic paper based analytical device implementing ion concentration polarization (ICP) for rapid pre‐concentration of Escherichia coli in water. The fabricated device consists of a paper channel with a Nafion ® membrane and in‐built micro wire electrodes to supply electric voltage to induce the ICP effect. E. coli cells were stained with SYTO 9 and fluorescence was used as a sensing method. The device achieved high concentration factor up to 2 × 10 5 within minutes. The effect of total ion concentration, on ICP and fluorescence intensity was studied. The reported device and method are suitable and effective for detection of E. coli during ballast water quality monitoring, coastal water quality monitoring where high salinity water is present.