Premium
Ion pair assisted micro matrix solid phase dispersion extraction of alkaloids from medical plant
Author(s) -
Dong Xin,
Yang Juan,
Zheng XiaoTing,
Chen Yan,
Ye LiHong,
Wang Bin,
Zheng Hui,
Cao Jun,
Wang QiuYan,
Hu YuHan
Publication year - 2020
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900244
Subject(s) - solid phase extraction , chromatography , elution , extraction (chemistry) , chemistry , solvent , reagent , cryptand , matrix (chemical analysis) , sorbent , repeatability , analytical chemistry (journal) , ion , adsorption , organic chemistry
A novel micro matrix solid phase dispersion method was successfully used for the extraction of quaternary alkaloids in Phellodendri chinensis cortex . The elution of target compounds was accomplished with sodium hexanesulfonate as the eluent solvent. A neutral ion pair was formed between ion‐pairing reagent and positively charged alkaloids in this process, which was beneficial for selectively extraction of polar alkaloids. Several parameters were optimized and the optimal conditions were listed as follows: silica gel as the sorbent, silica to sample mass ratio of 1:1, the grinding time of 1 min. The exhaustive elution of targets was achieved by 200 µL methanol/water (9:1) containing 150 mM sodium hexane sulfonate at pH 4.5. The method validation covered linearity, recovery, precision of intraday and interday, limits of detection, limits of quantitation, and repeatability. This established method was rapid, simple, environmentally friendly, and highly sensitive.