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Analysis of thrombin‐antithrombin complex formation using microchip electrophoresis and mass spectrometry
Author(s) -
Nielsen Jacob B.,
Nielsen Anna V.,
Carson Richard H.,
Lin HsienJung L.,
Hanson Robert L.,
Sonker Mukul,
Mortensen Daniel N.,
Price John C.,
Woolley Adam T.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900235
Subject(s) - thrombin , antithrombin , mass spectrometry , chromatography , biomarker , chemistry , gel electrophoresis , blot , thrombin generation , medicine , biochemistry , platelet , heparin , gene
Preterm birth (PTB) related health problems take over one million lives each year, and currently, no clinical analysis is available to determine if a fetus is at risk for PTB. Here, we describe the preparation of a key PTB risk biomarker, thrombin‐antithrombin (TAT), and characterize it using dot blots, MS, and microchip electrophoresis (µCE). The pH for fluorescently labeling TAT was also optimized using spectrofluorometry and spectrophotometry. The LOD of TAT was measured in µCE. Lastly, TAT was combined with six other PTB risk biomarkers and separated in µCE. The ability to make and characterize TAT is an important step toward the development of an integrated microfluidic diagnostic for PTB risk.