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Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples
Author(s) -
Lačná Júlia,
Přikryl Jan,
Teshima Norio,
Murakami Hiroya,
Esaka Yukihiro,
Foret František,
Kubáň Petr
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900112
Subject(s) - chemistry , capillary electrophoresis , chromatography , electrolyte , analyte , electrophoresis , detection limit , hydroxymethyl , glutathione , capillary action , conductometry , fluorescence spectroscopy , analytical chemistry (journal) , fluorescence , electrode , organic chemistry , materials science , physics , quantum mechanics , composite material , enzyme
In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C 4 D‐LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2‐[4‐(2‐hydroxyethyl)piperazin‐1‐yl]ethanesulfonic acid, 15 mM 2‐amino‐2‐hydroxymethyl‐propane‐1,3‐diol, and 2 mM 18‐crown‐6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double‐opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C 4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.