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Baseline separation of α and β‐acids homologues and isomers in hop ( Humulus lupulus L .) by CD‐MEKC‐UV
Author(s) -
Duarte Lucas M.,
Amorim Tatiane L.,
Adriano Luiz H. C.,
Oliveira Marcone A. L.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900101
Subject(s) - humulus lupulus , hop (telecommunications) , repeatability , chemistry , chromatography , sodium dodecyl sulfate , acetonitrile , sodium , electrolyte , analytical chemistry (journal) , organic chemistry , computer network , electrode , computer science
An alternative method for simultaneous baseline separation of α and β‐acids homologues and isomers in hop by CD‐MEKC with UV detection was proposed. The optimized background electrolyte was composed of 30 mmol/L sodium tetraborate solution, 45 mmol/L sodium dodecyl sulfate, 20 mmol/L β‐cyclodextrin and 10% v/v acetonitrile. The instrumental conditions were evaluated by using a 3 3 Box‐Benhken experimental design. In order to demonstrate the applicability of the method, 21 hop samples from different varieties were analyzed. The repeatability intra‐ and interday tests were performed and relative standard deviations lower than 7% for area and migration times were observed. The present method comprehended 8 min analysis time and revealed to be faster and more efficient when compared to previous reports from literature.