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A micellar electrokinetic chromatography approach using diastereomeric derivatization and a volatile surfactant for the enantioselective separation of selenomethionine
Author(s) -
PérezMíguez Raquel,
Marina Maria Luisa,
CastroPuyana María
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900077
Subject(s) - derivatization , chromatography , chemistry , chloroformate , diastereomer , reagent , enantioselective synthesis , micellar electrokinetic chromatography , ethyl chloroformate , enantiomer , resolution (logic) , detection limit , high performance liquid chromatography , organic chemistry , artificial intelligence , computer science , catalysis
A MEKC methodology with UV detection was developed for the enantioselective separation of selenomethionine (SeMet). The use of (+)‐1‐(9‐fluorenyl)ethyl chloroformate (FLEC) as chiral derivatization reagent to form SeMet diastereomers enabled their subsequent separation using ammonium perfluorooctanoate (APFO) as a volatile pseudostationary phase. The effect of APFO concentration and pH, temperature, injection volume, and derivatization conditions (time and FLEC/SeMet ratio) were evaluated in order to select the best separation conditions. A chiral resolution of 4.4 for DL‐SeMet was achieved in less than 6 min using 100 mM APFO at pH 8.5 as electrophoretic buffer. Satisfactory results were obtained in terms of linearity, precision (RSD from 3.4 to 5.1% for migration times and from 1.8 to 4.6% for corrected peak areas), accuracy, and LODs (3.1 × 10 −6 M and 3.7 × 10 −6 M for d and l enantiomers, respectively). The method was successfully applied to the determination of l ‐SeMet in food supplements.