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Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones
Author(s) -
Chen Yuanbo,
Jiang Bo,
Hu Yechen,
Deng Nan,
Zhao Baofeng,
Li Xiao,
Liang Zhen,
Zhang Lihua,
Zhang Yukui
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900061
Subject(s) - aptamer , graphene , polyethylenimine , oxide , histone , chemistry , combinatorial chemistry , nanoparticle , nanocomposite , lysine , nanotechnology , materials science , dna , organic chemistry , biochemistry , amino acid , microbiology and biotechnology , biology , transfection , gene
Abstract The binding coverage of aptamer was an important restricted factor for aptamer‐based affinity enrichment strategy for capturing target molecules. Herein, we designed and prepared aptamer functionalized graphene oxide based nanocomposites (GO/NH 2 ‐NTA/Fe 3 O 4 /PEI/Au), and the coverage density of aptamer was high to 33.1 nmol/mg. The high aptamer coverage density was contributed to the large surface area of graphene oxide. The successive modification of Nα,Nα‐Bis(carboxymethyl)‐L‐lysine, magnetic nanoparticles, polyethylenimine, and Au nanoparticles ensured the histone purification with fast speed and high purity. Histones could be captured rapidly and specifically from nucleoproteins by our aptamer based purification strategy, while traditional acid‐extraction could not specifically enrich histones. Compared with traditional acid‐extraction method, rapid and efficient discovery of histones and their post‐translational modifications, such as several kinds of methylation at H3.1K9 and H3.1K27, were achieved confidently. It demonstrated that our aptamer functionalized magnetic graphene oxide nanocomposites have a great potential for histone analysis.