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In‐tube solid‐phase microextraction capillary column packed with mesoporous TiO 2 nanoparticles for phosphopeptide analysis
Author(s) -
Li Ke,
Zhao Shuo,
Yan Yong,
Zhang Dongtang,
Peng Manhua,
Wang Yanan,
Guo Guangsheng,
Wang Xiayan
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900055
Subject(s) - phosphopeptide , mesoporous material , solid phase microextraction , chromatography , monolith , capillary action , nanoparticle , materials science , packed bed , chemistry , solid phase extraction , casein , selectivity , extraction (chemistry) , mass spectrometry , nanotechnology , gas chromatography–mass spectrometry , catalysis , peptide , organic chemistry , biochemistry , composite material
Abstract In this study, an in‐tube solid‐phase microextraction column packed with mesoporous TiO 2 nanoparticles, coupled with MALDI–TOF–MS, was applied to the selective enrichment and detection of phosphopeptides in complex biological samples. The mesoporous TiO 2 nanoparticles with high specific surface areas, prepared by a sol–gel and solvothermal method, were injected into the capillary using a slurry packing method with in situ polymerized monolithic segments as frits. Compared with the traditional solid‐phase extraction method, the TiO 2 ‐packed column with an effective length of 1 cm exhibited excellent selectivity ( α ‐casein/ β ‐casein/BSA molar ratio of 1:1:100) and sensitivity (10 fmol of a β ‐casein enzymatic hydrolysis sample) for the enrichment of phosphopeptides. These performance characteristics make this system suitable for the detection of phosphorylated peptides in practical biosamples, such as nonfat milk.