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One‐pot synthesis of highly fluorescent silicon nanoparticles for sensitive and selective detection of hemoglobin
Author(s) -
Li Quan,
Peng Kaite,
Yu Yingchun,
Ruan Xiangyan,
Wei Yun
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201900023
Subject(s) - fluorescence , ethylenediamine , nanoparticle , quenching (fluorescence) , chemistry , quantum yield , silicon , hemoglobin , linear range , materials science , nuclear chemistry , detection limit , inorganic chemistry , nanotechnology , chromatography , organic chemistry , physics , quantum mechanics
In this work, a simple, selective, and sensitive probe for hemoglobin based on the quenched fluorescence of silicon nanoparticles (SiNPs) was fabricated. The SiNPs were synthesized by a simple hydrothermal treatment from N‐[3‐(trimethoxysilyl)propyl]ethylenediamine and sodium citrate. The as‐prepared SiNPs exhibited good water‐solubility and high fluorescence with the quantum yield of 70%. The fluorescence of the SiNPs could be remarkably quenched by hemoglobin. A wide linear range was obtained from 50 nM to 4000 nM with a LOD of 40 nM. The quenching mechanism was investigated by UV‐Vis absorption spectrometry and time‐resolved fluorescence spectrometry.