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Front Cover: Streamlined microfluidic analysis of phosphopeptides using stable isotope‐labeled synthetic peptides and MRM‐MS detection
Author(s) -
Deng J.,
Ikenishi F.,
Smith N.,
Lazar I. M.
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201870201
Subject(s) - phosphorylation , chemistry , mass spectrometry , cell growth , microfluidics , cell , schematic , cover (algebra) , cell cycle , front cover , microbiology and biotechnology , biophysics , chromatography , nanotechnology , biochemistry , biology , materials science , mechanical engineering , electronic engineering , engineering
DOI: 10.1002/elps.201800133 The front cover picture shows a schematic representation of a cell that is ushered into the cell cycle in the presence of growth factors, such as EGF, that bind to cell surface EGFR/ERBB2 receptors. The binding of the growth factors initiates signaling processes transduced via protein phosphorylation into the nucleus, to lead ultimately to excessive cell proliferation (backdrop of proliferating cells). Mass spectrometry (lower left inset) and microfluidics (upper right inset) represent a powerful platform for the fast analysis and quantification of signaling phosphopeptides (bottom mass spectrum) and for exploring cellular functions mediated via protein phosphorylation.