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Back Cover: How many proteins can be identified in a 2DE gel spot within an analysis of a complex human cancer tissue proteome?
Author(s) -
Zhan Xianquan,
Yang Haiyan,
Peng Fang,
Li Jianglin,
Mu Yun,
Long Ying,
Cheng Tingting,
Huang Yuda,
Li Zhao,
Lu Miaolong,
Li Na,
Li Maoyu,
Liu Jianping,
Jungblut Peter R.
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201870052
Subject(s) - proteome , computational biology , biology , spots , human proteome project , two dimensional gel electrophoresis , proteomics , human proteins , chemistry , biochemistry , gene , botany
DOI: 10.1002/elps.201700330 The cover picture shows each 2‐DE spot contains many proteins (over 50 to several hundreds) in analysis of human complex cancer tissue proteome, and most of proteins are low‐abundance proteins. Multiple proteins per spot confirm the necessity of isotopic labeling in large‐scale quantification of different protein species in a proteome. Many proteins were present in several of the analyzed spots showing the ability of 2‐DE‐MS to separate at the protein species level. 2‐DE coupled with high‐sensitivity LC‐MS is a powerful approach to detect, identify and quantify protein species, and exceeds the resolution power of bottom‐up LC‐MS.