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Recent advances and challenges in the recovery and purification of cellular exosomes
Author(s) -
AyalaMar Sergio,
DonosoQuezada Javier,
GalloVillanueva Roberto C.,
PerezGonzalez Victor H.,
GonzálezValdez José
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800526
Subject(s) - exosome , microvesicles , nanocarriers , context (archaeology) , computer science , isolation (microbiology) , computational biology , nanotechnology , risk analysis (engineering) , biochemical engineering , drug delivery , biology , bioinformatics , microrna , medicine , engineering , materials science , paleontology , biochemistry , gene
Exosomes are nanovesicles secreted by most cellular types that carry important biochemical compounds throughout the body with different purposes, playing a preponderant role in cellular communication. Because of their structure, physicochemical properties and stability, recent studies are focusing in their use as nanocarriers for different therapeutic compounds for the treatment of different diseases ranging from cancer to Parkinson's disease. However, current bioseparation protocols and methodologies are selected based on the final exosome application or intended use and present both advantages and disadvantages when compared among them. In this context, this review aims to present the most important technologies available for exosome isolation while discussing their advantages and disadvantages and the possibilities of being combined with other strategies. This is critical since the development of novel exosome‐based therapeutic strategies will be constrained to the effectiveness and yield of the selected downstream purification methodologies for which a thorough understanding of the available technological resources is needed.