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Stiffness measurement of nanosized liposomes using solid‐state nanopore sensor with automated recapturing platform
Author(s) -
Lee Jung Soo,
Saharia Jugal,
Bandara Y. M. Nuwan D. Y.,
Karawdeniya Buddini Iroshika,
Goyal Gaurav,
Darvish Armin,
Wang Qingxiao,
Kim Moon J.,
Kim Min Jun
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800476
Subject(s) - nanopore , voltage , materials science , stiffness , liposome , nanotechnology , analyte , vesicle , particle (ecology) , biological system , membrane , chemistry , composite material , chromatography , electrical engineering , engineering , oceanography , biochemistry , geology , biology
This paper describes a method to gauge the stiffness of nanosized liposomes – a nanoscale vesicle – using a custom‐made recapture platform coupled to a solid‐state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re‐translocate the same analyte through the nanopore – provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i.e., 100 mV) permitting higher recapture instances than at higher voltages (>200 mV) since the probability of particles exiting the nanopore capture radius increases with voltage. The shape deformation was inferred by comparing the normalized relative current blockade (Δ I / I 0 ̂ )at the two voltage polarities to that of a rigid particle, i.e., polystyrene beads. We found that liposomes deform to adopt a prolate shape at higher voltages. This platform can be further applied to investigate the stiffness of other types of soft matters, e.g., virus, exosomes, endosomes, and accelerate the potential studies in pharmaceutics for increasing the drug packing and unpacking mechanism by controlling the stiffness of the drug vesicles.