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Resolving quantum dots and peptide assembly and disassembly using bending capillary electrophoresis
Author(s) -
Wang Jianhao,
Zhu Zhilan,
Jia Wenjing,
Qiu Lin,
Chang Yufeng,
Li Jie,
Ma Luping,
You Ying,
Wang Jianpeng,
Liu Li,
Xia Jiang,
Liu Xiaoqian,
Li YongQiang,
Jiang Pengju
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800466
Subject(s) - quantum dot , biomolecule , capillary electrophoresis , peptide , capillary action , nanoparticle , nanotechnology , bending , chemistry , biophysics , materials science , chromatography , biology , biochemistry , composite material
Despite the numerous techniques developed for the studying nanoparticle and peptide interaction nowadays, sensitive and convenient assay in the process of flow, especially to simulate the self‐assembly of quantum dots (QDs) and peptide inflow in blood vessels, still remains big challenges. Here, we report a novel assay for studying the self‐assembly of QDs and peptide, based on CE using a bending capillary. We demonstrate that the semicircles numbers of the bending capillary affect the self‐assembly kinetics of CdSe/ZnS QDs and ATTO‐D 3 LVPRGSGP 9 G 2 H 6 peptide. Moreover, benefitting from this novel assay, the effect of the position on the self‐assembly has also been realized. More importantly, we also demonstrate that this novel assay can be used for studying the stability of the QDs–peptide complex inflow. We believe that our novel assay proposed in this work could be further used as a general strategy for the studying nanoparticle–biomolecule interaction or biomolecule–biomolecule interaction.