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Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems
Author(s) -
Sharifi Noghabi Hamideh,
Soo Mandy,
Khamenehfar Avid,
Li Paul C.H.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800451
Subject(s) - single cell analysis , rhodamine 123 , inverted microscope , microfluidics , fluorescence , cell , efflux , fluorescence microscope , chemistry , dielectrophoresis , microscope , rhodamine , biophysics , materials science , nanotechnology , multiple drug resistance , biology , biochemistry , pathology , optics , antibiotics , medicine , physics
Here, we report a microfluidic same‐single‐cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single‐cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct dielectrophoretic cell trapping together with the compact SCB. By using the DEP force, a single acute myeloid leukemia cell was trapped in the cell retention structure of the chip. This allowed us to detect dye accumulation in the MDR leukemic cells in the presence of cyclosporine A (CsA). CsA and rhodamine 123 were used as the P‐glycoprotein inhibitor and fluorescent dye, respectively. The result showed that the Rh123 fluorescence signal in a single‐cell increased dramatically over its same‐cell control on both fluorescence detection systems due to the inhibition by CsA.