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A smartphone‐based on‐site nucleic acid testing platform at point‐of‐care settings
Author(s) -
Xu Xiayu,
Wang Xuemin,
Hu Jie,
Gong Yang,
Wang Lin,
Zhou Wan,
Li XiuJun,
Xu Feng
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800449
Subject(s) - nucleic acid , nat , point of care , point of care testing , android app , computer science , homogeneous , android (operating system) , smartphone application , nucleic acid detection , chemistry , mathematics , medicine , pathology , operating system , multimedia , biochemistry , computer network , combinatorics
We developed a smartphone‐based on‐site nucleic acid testing (NAT) platform that can image and analyze lateral flow nucleic acid assays at point‐of‐care settings. An inexpensive add‐on was devised to run lateral flow assays while providing homogeneous ambient light for imaging. In addition, an Android app with a user‐friendly interface was developed for the result analysis and management. Linear color calibration is implemented inside the app to minimize the colorimetric reaction difference between smartphones. A relationship function between nucleic acid concentration and colorimetric reaction was established and evaluated by leave‐one‐out cross validation. The predicted concentration and true concentration showed a good agreement with an R‐squared value of 0.96. This smartphone‐based NAT platform can be used to diagnose infectious diseases and monitor disease progression, and assess treatment efficacy, especially for resource‐limited settings.