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Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertion/deletion polymorphisms
Author(s) -
Jin XiaoYe,
Wei YuanYuan,
Cui Wei,
Chen Chong,
Guo YuXin,
Zhang WenQing,
Zhu BoFeng
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800412
Subject(s) - indel , forensic identification , genetics , biology , multiplex polymerase chain reaction , multiplex , indel mutation , forensic science , polymerase chain reaction , population , amplicon , computational biology , gene , single nucleotide polymorphism , genotype , medicine , environmental health
Insertion/deletion (InDel) polymorphisms have been widely used in the fields of population genetics, genetic map constructions, and forensic investigations owing to the advantages of their low mutation rates, widespread distributions in the human genome, and small amplicon sizes. In order to provide more InDels with high discrimination power in Chinese populations, we selected and constructed one novel multiplex PCR‐InDel panel for forensic individual identification. Genetic distributions of these 35 InDels in five reference populations from East Asia showed low genetic differentiations among these populations. Forensic efficiency evaluations of these InDels revealed that these loci could perform well for forensic individual identifications in these reference populations. In the meantime, genetic diversities and forensic parameters of these InDels were further investigated in the studied Kazak group. Mean value of polymorphism information content for 35 InDels was 0.3611. Cumulative power of discrimination of 35 InDels was 0.603 in Kazak group. Given these results, the panel is suitable for individual identifications in the studied Kazak and these reference populations.